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5′-cap modification by VCE. (A) The capping reaction catalyzed by VCE. VCE adds GTP (above, red) to the 5′ end of the transcript (left, blue) via triphosphate. Then, a methyl group is transferred from SAM (S-Adenosyl methionine, green) to the N7 of guanine. (B) Chemical structures of the GTP analogs used in this study. Modified moieties are indicated in red. See Table 1 for unabbreviated names.
