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. 2023 Feb 3;51(6):e34. doi: 10.1093/nar/gkad019

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Capping with chemically-modified GTP analogs by VCE. (A) Outline of the experiment. Short RNA was reacted with VCE in the presence of a GTP analog and SAM. The reaction product was applied to electrophoresis to observe the capped and uncapped fractions. (B) A representative example of the results. Capped products were clearly distinguished from uncapped RNA. (C) Capping (GMP-incorporation) efficiencies of each GTP analog in standard conditions. Grey (left) and black (right) bars indicate the results for 1 h and 24 h reactions, respectively. (D) Capping efficiencies in modified conditions. (E) N7-methylation efficiencies analyzed with LC–MS.