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. 2023 Feb 3;51(6):e34. doi: 10.1093/nar/gkad019

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Translational activities of cap-modified mRNAs. (A) Fluorescence images of hmAG1 expressed from cap-modified mRNAs in HeLa cells. Scale bars, 200 μm. (B) The expression level of hmAG1 in HeLa cells as analyzed with flow cytometry. The values were normalized by the expression level of iRFP670 co-transfected as a transfection control. (C) Fluorescence images of hmAG1 expressed from cap-modified mRNAs in HEK293FT cells. Scale bars, 200 μm. (D) The expression level of hmAG1 in HEK293FT cells as analyzed with flow cytometry. The values were normalized by the expression level of iRFP670 co-transfected as a transfection control. Statistical significance to m⁷G-capped RNA (#1): n.s.: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001 (Dunnett's test).