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. 2023 Feb 3;51(6):e34. doi: 10.1093/nar/gkad019

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Time-course changes in expressed protein level and the amount of the residual mRNA in HeLa cells. (A) The expressed hmAG1 level was analyzed with flow cytometry. The mean of fluorescent intensity at each time point is shown. (B) The relative amount of residual mRNA after transfection was quantified by real-time qPCR. The black point indicates the mRNA level of natural m⁷G (#1)-capped mRNA. Each value was normalized to that of m⁷G (#1)-capped mRNA at 4 h.