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. 2023 Feb 3;51(6):e34. doi: 10.1093/nar/gkad019

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Bioconjugation using azide-containing modified caps. (A) The SPAAC reaction was used to introduce functional molecules to the azide group at the 5′ cap of RNA. (B) Fluorescent dye- or biotin-labelling of short RNA. Modified caps with the azide group were specifically labelled. (C) Confocal microscopic images of HeLa cells transfected with AF647-labelled hmAG1 mRNA. Scale bars, 100 μm. Images in the right columns are magnifications (100 μm × 100 μm) of the boxed regions in the adjacent columns.