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. 2023 Feb 28;69(2):109–117. doi: 10.1262/jrd.2022-123

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Fig. 2. — Cryoprotective effects of trehalose in a combination of DMSO or PG on the post-thaw recovery and viability of cultured PGCs. Cultured PGCs were cryopreserved with different concentrations of trehalose (0–0.5 M) in combination with (A and B) 5% DMSO or (C and D) 7.5% PG. (A and C) Recovery rates of total PGCs, including dead (light gray) and viable cells (white) after cryopreservation with each cryoprotectant solution. (B and D) Viability of PGCs recovered after freeze-thaw with each cryoprotectant solution. All data are presented as mean ± SEM. Data with different letters are significantly different (P < 0.05).