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. 2023 Feb 28;69(2):109–117. doi: 10.1262/jrd.2022-123

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Fig. 4. — Additive effect of trehalose and chicken serum on the post-thaw recovery and viability of cultured PGCs. Cultured PGCs were cryopreserved in 5% DMSO or 7.5% PG solutions either alone or combined with trehalose and/or chicken serum. (A and C) Recovery rates of total PGCs, including dead (light gray) and viable cells (white), thawing with each cryoprotectant solution. (B and D) Viability of PGCs recovered after freeze-thaw with each cryoprotectant solution. All data are presented as mean ± SEM. Data with different letters are significantly different (P < 0.05).