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. 2023 Feb 28;69(2):109–117. doi: 10.1262/jrd.2022-123

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Fig. 6. — Revival of a rare chicken breed derived from cryopreserved PGCs. (A) Female and male Kurokashiwa. This rare breed was used to verify the utility of DTS and PTS cryomedia for cryobanking of PGCs in (B)–(F). (B) Percentage of donor-derived offspring from White Leghorn (WL) recipient roosters transplanted with frozen-thawed PGCs of Kurokashiwa by mating with normal Barred Plymouth Rock (BPR) hens. (C) Percentage of donor-derived offspring by crossing female and male WL recipients. (D) Kurokashiwa offspring (black feather) revived from WL recipient parents (white feather) by the transplantation of frozen-thawed PGCs. (E) Recovery and (F) viability rates of a female PGC line (K5) of Kurokashiwa after storage in liquid nitrogen for > 2.5 years. Bars with light gray and white indicate total cells and viable cells, respectively. Values in (E) and (F) are shown as mean ± SEM. Data with different letters are significantly different (P < 0.05).