KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| rabbit anti-ARID1A | Abcam | Cat#: ab182560 |
| rabbit anti-ARID1B | Cell Signaling | Cat#: 92964; RRID:AB_2800195 |
| rabbit anti-BRG1 | Cell Signaling | Cat#: 49360; RRID:AB_2728743 |
| rabbit anti-SNF5 | Cell Signaling | Cat#: 91735; RRID:AB_2800172 |
| rabbit anti-HMGCS1 | Proteintech | Cat#: 17643–1-AP; RRID:AB_2248359 |
| mouse anti-HMGCR | Thermo Fisher | Cat#: CL0259; RRID:AB_2786972 |
| rabbit anti-MVD | Proteintech | Cat#: 15331–1-AP; RRID:AB_2147439 |
| mouse anti-FDPS | Proteintech | Cat#: 16129–1-AP; RRID:AB_2104749 |
| rabbit anti-SREBP2 | Proteintech | Cat#: 28212–1-AP; RRID:AB_2881091 |
| rabbit anti-HMGB1 | Abcam | Cat#: ab18256; RRID:AB_444360 |
| rabbit anti-cleaved IL-1β | Cell Signaling | Cat#: 83186; RRID:AB_2800010 |
| rabbit anti-GSDMD | Abcam | Cat#: ab210070; RRID:AB_2893325 |
| rabbit anti-caspase1 | Cell Signaling | Cat#: 3866; RRID:AB_2069051 |
| rabbit anti-cleaved caspase1 | Biovision | Cat#: A1004 |
| mouse anti-Histone H3 | Cell Signaling | Cat#: 14269; RRID:AB_2756816 |
| rabbit anti-α-tubulin | Cell Signaling | Cat#: 2125; RRID:AB_2619646 |
| mouse anti-β-actin | Sigma-Aldrich | Cat#: A5316; RRID:AB_476743 |
| rabbit anti-SNF5 | Bethyl | Cat#: A301–087A; RRID:AB_2191714 |
| mouse anti-Pol II | Santa Cruz | Cat#: sc-47701; RRID:AB_677353 |
| anti-mouse IgG, HRP-linked antibody | Cell Signaling | Cat#: 7076; RRID:AB_330924 |
| anti-rabbit IgG, HRP-linked antibody | Cell Signaling | Cat#: 7074; RRID:AB_2099233 |
| mouse anti-cGAS | Santa Cruz | Cat#: sc-515777; RRID:AB_2734736 |
| rabbit anti-γH2AX | Cell Signaling | Cat#: 9718; RRID:AB_2118009 |
| rabbit anti-cleaved PARP p85 | Cell Signaling | Cat#: 5625; RRID:AB_10699459 |
| mouse anti-RAC1 | Proteintech | Cat#: 66122–1-Ig; RRID:AB_2883264 |
| rabbit anti-RHOA | Proteintech | Cat#: 10749–1-AP; RRID:AB_2285104 |
| rabbit anti-HNF1β | Sigma-Aldrich | Cat#: HPA002083; RRID:AB_1080232 |
| rabbit anti-cleaved Caspase 3 | Cell Signaling | Cat#: 9661L; RRID:AB_2341188 |
| rabbit anti-Histone H3K4me3 | Active Motif | Cat#: 39159; RRID:AB_2615077 |
| rabbit anti-Histone H3K27ac | Abcam | Cat#: ab4729; RRID:AB_2118291 |
| IgG isotype control | Bio X Cell, | Cat#: BE0090; RRID:AB_1107780 |
| mouse PD-L1 antibody for in vivo | Bio X Cell | Cat#: BE0101; RRID:AB_10949073 |
| human PD-L1 antibody for in vivo | R&D Systems | Cat#: MAB10348 |
| Fc blocking anti-mouse CD16/CD32 | BD Bioscience | Cat#: 553142; RRID:AB_394657 |
| PE-Cy7 anti-mouse CD3e | BD Bioscience | Cat#: 552774; RRID:AB_394460 |
| BV711 anti-mouse CD45 | Biolegend | Cat#: 103147; RRID:AB_2564283 |
| PE/Cyanine7 anti-mouse PD-1 | Biolegend | Cat#: 109109; RRID:AB_572016 |
| BV421 anti-mouse LAG-3 | Biolegend | Cat#: 125221; RRID:AB_2572080 |
| Alexa Fluor 700 rat anti-mouse CD45 | BD Biosciences | Cat#: 560510; RRID:AB_1645208 |
| BB515 mouse anti-human CD45 | BD Biosciences | Cat#: 564586; RRID:AB_2869588 |
| BUV805 mouse anti-human CD3 | BD Biosciences | Cat#: 612895; RRID:AB_2870183 |
| BUV395 mouse anti-human CD4 | BD Biosciences | Cat#: 563550; RRID:AB_2738273 |
| PerCP-Cy5.5 mouse anti-human CD8 | BD Biosciences | Cat#: 565310; RRID:AB_2687497 |
| BV786 mouse anti-human PD-1 | BD Biosciences | Cat#: 563789; RRID:AB_2738425 |
| BV421 mouse anti-human LAG-3 | BD Biosciences | Cat#: 565720; RRID:AB_2744330 |
| PerCP/Cyanine5.5 anti-mouse CD19 | Biolegend | Cat#: 115534; RRID:AB_2072925 |
| APC/Fire750 anti-mouse CD8b.2 | Biolegend | Cat#: 140420; RRID:AB_2819885 |
| Alexa Fluor 647 anti-mouse Ki67 | Biolegend | Cat#: 652408; RRID:AB_2562139 |
| APC anti-mouse Arg1 | eBioscience | Cat#: 17369780; RRID:AB_2734834 |
| APC-Cyanine7 anti-mouse CD64 | Biolegend | Cat#: 139334; RRID:AB_2910296 |
| FITC anti-mouse Lyve1 | eBioscience | Cat#: 53-0443-82; RRID:AB_1633415 |
| BUV805 anti-mouse CD11b | BD Biosciences | Cat#: 741934; RRID:AB_2871246 |
| BV421 anti-mouse NK1.1 | Biolegend | Cat#: 108732; RRID:AB_2562218 |
| BV711 anti-mouse Ly6G | Biolegend | Cat#: 127643; RRID:AB_2565971 |
| BV785 anti-mouse Ly6C | Biolegend | Cat#: 128041; RRID:AB_2565852 |
| PE anti-mouse CD163 | Biolegend | Cat#: 156704; RRID:AB_2860724 |
| PE-Dazzle594 anti-mouse CD169 | Biolegend | Cat#: 142424; RRID:AB_2750059 |
| PE-Cyanine7 anti-mouse F4/80 | Biolegend | Cat#: 123114; RRID:AB_893478 |
| Bacterial and virus strains | ||
| DH5α competent E. coli | Thermo Fisher | Cat#: 18265017 |
| Stellar competent E. coli | Fisher | Cat#: DAL1100 |
| Chemicals, peptides, and recombinant proteins | ||
| simvastatin | Sigma-Aldrich | Cat#: S6196 |
| atorvastatin | Sigma-Aldrich | Cat#: PHR1422 |
| etoposide | Sigma-Aldrich | Cat#: E1383 |
| methyl-β-cyclodextrin | Cayman Chemical | Cat#: 21633 |
| Nigericin | InvivoGen | Cat#: tlrl-nig |
| mevalonate | Sigma-Aldrich | Cat#: 43987 |
| GGPP | Sigma-Aldrich | Cat#: G6025 |
| cholesterol | Sigma-Aldrich | Cat#: C4951 |
| lipofectamine 2000 | Thermo Fisher | Cat#: 11668027 |
| AlamarBlue reagent | Fisher | Cat#: DAL1100 |
| chemiluminescent substrate | Thermo Fisher | Cat#: 34094 |
| protease inhibitors cocktail | Roche | Cat#: 11836170001 |
| micrococcal nuclease | Cell Signaling | Cat#: 10010 |
| Trizol reagent | Thermo Fisher | Cat#: 15596026 |
| prolong antifade reagent | Thermo Fisher | Cat#: P36961 |
| RedDot nuclear stain | Biotium | Cat#: 40061 |
| Filipin III | Sigma-Aldrich | Cat#: F4767 |
| mammalian protein extraction reagent | Thermo Fisher | Cat#: 78501 |
| Cre recombinase expressing-adenovirus | Vector Viral Core | Cat#: VVC-U of Iowa-5 |
| red blood cell lysis buffer | Thermo Fisher | Cat#: 00-4333-57 |
| Fixable Viability Stain 510 | BD Biosciences | Cat#: 564406; RRID:AB_2869572 |
| Mayer’s Hematoxylin | Dako | Cat#: S3309 |
| Critical commercial assays | ||
| LookOut Mycoplasma PCR detection | Sigma-Aldrich | Cat#: MP0035 |
| ChIP DNA clean and concentrator kit | Zymo Research | Cat#: D5205 |
| Reverse Transcription kit | Thermo Fisher | Cat#: 4368813 |
| Cholesterol Assay Kit | Abcam | Cat#: ab133116 |
| Amplex Red Cholesterol Quantitation Kit | Thermo Fisher | Cat#: A12216 |
| 2’3’-cGAMP ELISA Kit | Cayman Chemical | Cat#: 501700 |
| FAM FLICA Caspase1 Kit | Bio-Rad | Cat#: ICT097 |
| Mouse Dissociation Kit | MiltenyiBiotec | Cat#: 130-096-730 |
| Viability staining Kit | Thermo Fisher | Cat#: L35957 |
| Dako EnVision+ system | Dako | Cat#: K4011 |
| HMG-CoA Reductase Assay Kit | Sigma-Aldrich | Cat#: CS1090 |
| Annexin V Conjugates for Apoptosis Detection | Thermo Fisher | Cat#: A35110 |
| Human IL-1β ELISA Kit | Abcam | Cat#: ab214025 |
| Human IL-18 ELISA Kit | Abcam | Cat#: ab215539 |
| True-Nuclea Transcription Factor Buffer Set | Biolegend | Cat#: 424401 |
| Experimental models: Cell lines | ||
| OVCA429 | Dr. Ie-Ming Shih, Johns Hopkins University | RRID:CVCL_3936 |
| RMG1 | JCRB | Cat# JCRB0172; RRID:CVCL_1662 |
| ES2 | ATCC | Cat#: CRL-1978; RRID: CVCL_3509 |
| SKOV3 | JCRB | Cat# NIHS0737; RRID:CVCL_4Y20 |
| OVISE | JCRB | Cat#: JCRB1043; RRID:CVCL_3116 |
| OVTOKO | JCRB | Cat#: NIHS0301; RRID:CVCL_3117 |
| TOV21G | ATCC | Cat#: CRL-11730; RRID:CVCL_3613 |
| HEK-293T | ATCC | Cat#: CRL-3216; RRID: CVCL_0063 |
| XVOA295 | PMID: 28737768 | N/A |
| G401 | PMID: 34085048 | N/A |
| 12Z | Dr. Ie-Ming Shih, Johns Hopkins University | RRID:CVCL_0Q73 |
| Experimental models: Organisms/strains | ||
| NSG mice | The Jackson Laboratory | Strain#: 005557; RRID:IMSR_JAX:005557 |
| Arid1a−/−;Pik3caH1047R transgenic mice | PMID: 28737768 | N/A |
| Oligonucleotides | ||
| Primers used in this study are listed in Table S2 | This paper | N/A |
| Recombinant DNA | ||
| pSpCas9(BB)-2A-GFP | Addgene | Cat#: 48138; RRID:Addgene_48138 |
| pFETCh_Donor | Addgene | Cat#: 63934; RRID:Addgene_63934 |
| pRP_ASC-LmCerulean | Addgene | Cat#: 41840; RRID:Addgene_41840 |
| plentiCRISPR v2 | Addgene | Cat#: 52961; RRID:Addgene_52961 |
| PlentiCRISPR-ARID1A | PMID: 30297712 | N/A |
| pLVX-M-puro | Addgene | Cat#: 125839; RRID:Addgene_125839 |
| pLVX-SNF5-puro | PMID: 34085048 | N/A |
| pCRISPR-Cre stuffer v4 | Addgene | Cat#: 158033; RRID:Addgene_158033 |
| pMD2.G | Addgene | Cat#: 12259; RRID:Addgene_12259 |
| psPAX2 | Addgene | Cat#: 12260; RRID:Addgene_12260 |
| pLKO.1-HMGCS1-shRNA1 | Molecular Screening Facility (Wistar) | TRCN0000045843 |
| pLKO.1-HMGCS1-shRNA2 | Molecular Screening Facility (Wistar) | TRCN0000045845 |
| pLKO.1-ARID1B-shRNA1 | Molecular Screening Facility (Wistar) | TRCN0000107361 |
| pLKO.1-ARID1B-shRNA2 | Molecular Screening Facility (Wistar) | TRCN0000107364 |
| pLKO.1-BRG1-shRNA1 | Molecular Screening Facility (Wistar) | TRCN0000015549 |
| pLKO.1- BRG1-shRNA2 | Molecular Screening Facility (Wistar) | TRCN0000015552 |
| pLKO.1-SNF5-shRNA1 | Molecular Screening Facility (Wistar) | TRCN0000039585 |
| pLKO.1-SNF5-shRNA2 | Molecular Screening Facility (Wistar) | TRCN0000039587 |
| pLKO.1-Caspase1-shRNA1 | Molecular Screening Facility (Wistar) | TRCN0000003502 |
| pLKO.1-Caspase1-shRNA2 | Molecular Screening Facility (Wistar) | TRCN0000003503 |
| pLKO.1-GSDMD-shRNA1 | Molecular Screening Facility (Wistar) | TRCN0000179394 |
| pLKO.1-GSDMD-shRNA2 | Molecular Screening Facility (Wistar) | TRCN0000180013 |
| Software and algorithms | ||
| GraphPad Prism version 8.0 | GraphPad Software Inc. | https://www.graphpad.com/ |
| FlowJo version 10.0 | FlowJo LLC | https://www.flowio.com/ |
| TraceFinder 4.1 | Thermo Fisher | https://www.thermofisher.com/order/catalog/product/OPTON-31001 |
| ImageJ version 1.53a | NIH | https://imagei.nih.gov/ii/ |
| GSEA version 4.1 | PMID: 16199517 | http://www.gsea-msigdb.org/gsea/index.isp |