Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 10;14:2003. doi: 10.1038/s41467-023-37786-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a, b EM density map (colored grey) of 2PQ fitted on S with all-down RBD (PDB: 6VXX) (a), and one-up RBD (PDB: 6VYB) (b). c The first differential curves for the relative fluorescence unit (RFU) from differential scanning fluorimetry with respect to temperature are shown for soluble 2PQ and 2P. Grey dotted line indicates the first apparent melting temperature (T_m) of 2P and 2PQ at approximately 46.5 °C; blue dotted line indicates the second apparent T_m of 2PQ at approximately 62 °C. d D994Q allows formation of an additional intraprotomer hydrogen bond as shown by structural modelling. Distinct protomers are in grey, light blue and pink. The Q758 and Q994 side chains are shown as sticks representation. Hydrogen bond is indicated in black dashed line with the distance indicated. e Titration of S bearing 2P or 2PQ mutations with an N-terminal domain (NTD), receptor-binding domain (RBD), first heptad repeat (HR1), or stem helix (SH) antibody via flow cytometry. Median fluorescence intensities (MFI) are shown as mean ± standard deviation from n = 3 independent replicates. Source data are provided as a Source Data file.