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. 2023 Apr 10;14:2020. doi: 10.1038/s41467-023-37785-2

Fig. 1. HIP CAR T cells are effective killers of Nalm6 tumor cells.

Fig. 1

a–c Flow cytometry of unedited (Mock, a) T cells, anti-CD19 allo CAR T cells (b), and anti-CD19 HIP CAR T cells (c) are shown. T cells are identified by CD4 or CD8 expression. Allo CAR T and HIP CAR T cells showed expression of the anti-CD19 CAR of approximately 70%. HIP CAR T cells showed disruption of the TCRαβ/CD3 complex and of HLA class I and II and overexpression of CD47 (representative graph of two independent experiments). d–f In vitro impedance cytotoxicity assay with Nalm6 target cells and HIP CAR T cells (d), allo CAR T cells (e), or Mock T cells (f) in different E:T ratios (mean ± SD, three independent replicates per group and time point).