Fig. 7. HIP CAR T cells provide lasting tumor control in fully immunocompetent allogeneic humanized mice.

a Humanized mice were injected with 1 × 10⁶ Luc⁺ Nalm6 cells and received 7 × 10⁶ allo CAR T cells or HIP CAR T cells on day 3 (one experiment). Spleen and bone marrow were taken after 27 or 56 days. b BLI images show the tumor burden for all mice in this study. c–e Graphs show BLI signals for animals in the HIP CAR T (c) and allo CAR T cell groups (d), and the Nalm6 only group (e, all single animals are shown). * indicates outliers that got assessed further. f, g The percentages of CAR⁺ cells in the bone marrow (f) and spleen (g) were assessed on day 56 (mean ± SD, n = 5 animals per group). Significant differences between the two CAR T cell groups are shown. h The percentage of CD19⁺ cells in the bone marrow was assessed on day 56 as endpoint (mean ± SD, n = 5 animals per group) and were significantly different. Endpoint values from earlier euthanized mice in the allo CAR T group due to high tumor burden were included in the day 56 graphs. i, j Reconstitution of human immune cells was assessed in the HIP CAR T (i) and the allo CAR T (j) groups after euthanasia. The percentage of hCD45⁺ cells among all CD45⁺ cells and the percentage of hCD3⁺ cells in the hCD45⁺ population was quantified (mean ± SD, n = 5 HIP animals, n = 3 allo animals with maintained hCD3⁺ population and n = 2 allo animals with lost hCD3⁺ population). k, l The percentages of CAR⁺ cells in the bone marrow (k) and spleen (l) were assessed separately for animals that had maintained their hCD3⁺ population (mean ± SD, n = 5 in the HIP CAR T group and n = 3 in the allo CAR T group). The 2 animals in the allo CAR T group that had lost their hCD3 population are shown separately. m The percentage of CD19⁺ cells in the bone marrow was assessed separately for animals that had maintained their hCD3⁺ population (n = 5 in the HIP CAR T group and n = 3 in the allo CAR T group, mean ± SD). The 2 animals in the allo CAR T group that had lost their hCD3 population are shown separately. For (f–h) and (k–m): Unpaired, two-tailed Student’s t test was used to compare HIP and allo CAR T cell groups and significant differences are shown.