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. 2023 Apr 10;14:2018. doi: 10.1038/s41467-023-37783-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–c BM analysis in young (5-6 month old) LepR-NTN1 mice demonstrating a decrease in BM cellularity a (N = 8 mice/group), and HSC frequency b, c (N = 6 mice/group), as compared to littermate controls. d, e Competitive HSCtransplantations (250 CD45.2+ donor HSCs with 10⁶ CD45.1 WBM competitor/recipient) demonstrating a loss of long-term (>6 months post-transplant) HSC engraftment potential d without lineage alterations e in HSCs derived from LepR-NTN1 mice (N = 6 donors/group; N = 18 Recipients for Control donors, N = 14 Recipients for LepR-NTN1 donors). f RT-qPCR analysis of FACS purified LepR+ MSCs demonstrating decreased NTN1 expression in LepR-NTN1 mice (N = 3 mice/group). g–i BM analysis of CDH5-NTN1 mice demonstrating no gross changes in BM cellularity g, and HSC frequency h, i, as compared to littermate controls (N = 5/group). j, k Competitive HSC transplantations (250 CD45.2+ donor HSCs with 10⁶ CD45.1 WBM competitor/recipient) demonstrating a loss of long-term engraftment potential j, along with a myeloid-biased output k, in HSCs derived from CDH5-NTN1 mice (N = 6 donors/group; N = 14 recipients/group). l RT-qPCR analysis of FACS purified BMECs demonstrating decreased NTN1 expression in CDH5-NTN1 mice (N = 3 mice/group). Error bars represent sample mean ± standard error of the mean (SEM). Statistical significance determined using two-tailed unpaired t-test. *P < 0.05; **P < 0.01; ***P < 0.001. ns denotes statistically not significant.