Figure 7.
In vivo anti-tumor efficacy of MBP NK in a solidified K562 tumor mouse model. (A) Experimental scheme of K562 xenograft mouse model. Female NIG mice, aged 7-8 weeks (n = 7 / group) were inoculated subcutaneously in their right flank with K562 tumor cells. When tumor volumes reached ~50 mm3, after 7 days of tumor inoculation, mice were intravenously administered MBP NK or NK-92 at a dose of 5 × 106 cells / mouse (3 doses total). (B) Tumor volume analysis to quantitatively determine tumor growth inhibition. Overall and individual responses are shown. Treatment of MBP NK resulted in potent tumor growth inhibition compared to NK-92 or IL-2 co-administrated NK-92. (C) A picture of tumors from each mouse after study termination. (D) On day 26, blood samples were collected from each mouse and IFN-γ level was analyzed by ELISA. (E) Body weight changes (%) in mice after injection of the indicated NK cells. All groups showed body weight change within the 5% range. The statistical significance was determined by ordinary one-way ANOVA with Holm-Šidák's multiple comparison test, ****, p < 0.0001. From all the analyses, statistically insignificant (ns) results are not shown.