Table 3.
Primer sequences were used for the real-time PCR analysis.
| Genes | Sequence 5′-3′ | GenBank number |
|---|---|---|
| GAPDH | F: GGAAAGTCATCCCTGAGCTGAAT | NM_204305.1 |
| R: GGCAGGTCAGGTCAACAACA | ||
| ZO-1 | F: AATACCTGACTGTCTTGCAG | XM_015278975.1 |
| R: TAAAGAAGGCTTTCCCTGAC | ||
| Occludin | F: GCAGATGTCCAGCGGTTACTAC | NM_205128.1 |
| R: CGAAGAAGCAGATGAGGCAGAG | ||
| Claudin | F: CATACTCCTGGGTCTGGTTGGT | NM_001013611.2 |
| R: GACAGCCATCCGCATCTTCT | ||
| IL-1β | F: ACTGGGCATCAAGGGCTA | XM_015297469 |
| R: GGTAGAAGATGAAGCGGGTC | ||
| IL-6 | F: GAAATCCCTCCTCGCCAATCT | XM_0152812832 |
| R: CCTCACGGTCTTCTCCATAAACG | ||
| TNF-α | F: TGTGTATGTGCAGCAACCCGTAGT | NM_204267 |
| R: GGCATTGCAATTTGGACAGAAGT | ||
| IFN-γ | F: AAAGCCGCACATCAAACACA | NM_205128.1 |
| R: GCCATCAGGAAGGTTGTTTTTC |