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. 2023 Mar 28;14:1116057. doi: 10.3389/fimmu.2023.1116057

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Copyright © 2023 Yan, Zhang, Chen, Lu, Huang, Cai, Chen, Bei, Bai, Lv, Fu and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relationship between immune cell subsets in tumor immune microenvironment and Amp11q13 status. Images of representative mIHC results (top, scale: 500 μm) were showed in two patients with and without Amp11q13. PD-1+ cells and FoxP3+ cells (bottom) in patients with Amp11q13 compared with nonAmp11q13 patients were also displayed. (A) Panel 1 referred to multiple stained slides and representative merged images show the distribution of individual markers [CD8, CD163, CD68, PD-1, PD-L1, pan-CK, DAPI for nucleic acids (blue)] and the separable vision of immunohistochemical staining with PD-1 antibody (green). (B) Panel 2 referred to multiple stained slides and representative merged images show the distribution of individual markers [CD3, CD20, FoxP3, CD56, CD4, pan-CK, DAPI for nucleic acids (blue)] and the separable vision of immunohistochemical staining with FoxP3 antibody (yellow).