Figure 3.

Memory T-cell phenotype in CD4⁺ and CD8⁺ T cells after the booster dose (T3). (A–D) T-cell phenotype in HCWs (n=15) and PwMS (n=22) in unstimulated condition. Frequency of CD4⁺ (A) and CD8⁺ T cells (C) gated according to CD45RA and CCR7 expression. Pie charts show the proportion of CD4⁺ (B) and CD8⁺ (D) T-cell subpopulations in both cohorts. Statistical analyses were performed using Mann-Whitney U test and p values <0.05 were considered significant. Friedman test followed by Dunn’s multiple comparison test was performed for pie charts analyses and pie graphs were generated using the median frequency. Red lines indicate medians. Each dot represents a different HCW or PwMS. HCWs, healthcare workers; PwMS, patients with multiple sclerosis; T_CM, central memory; T_EM, effector memory; T_EMRA, terminally differentiated effector memory.