Figure 5.

Ligilactobacillus murinus IRSD_2020 concentration is decreased in PS mouse faeces. The levels of the L. murinus strain IRSD_2020 (A) and of the reference L. murinus/animalis strain DSMZ 20602 (B) were quantified by TaqMan real-time PCR in the faeces of control mice (white) or PS mice (black), in the M (square) and F(circle) offspring. Data are expressed as scatter dot plot with the mean. Statistical analysis was performed using Mann-Whitney test. ****P<0.0001, significantly different from the control group (n=27–36 mice/group, three independent experiments). Spearman correlations were used to analyse the correlation between the faecal bacteria quantity and visceral motor response to colorectal distension expressed in AUC, in M (square) and F (circle) offspring. P and R values are indicated on each graph. AUC, area under the curve; F, female; M, male; PS, prenatal stress