Figure 6.

Ligilactobacillus murinus IRSD_2020 produces an analgesic lipopeptide. (A) Concentration of lipopeptides quantified by LC-MS/MS in the bacterial pellets of L. murinus IRSD_2020 cultivated without (white circle) or with (4 mg/mL) (black circle) GABA. Data are expressed as scatter dot plot with the mean (n=9). Statistical analysis was performed using Mann-Whitney test. *P<0.05, **P<0.01, significantly different from the corresponding L. murinus IRSD_2020 without GABA. (B) Concentration of lipopeptides quantified by LC-MS/MS in the colon of M (square) and F (circle) control (white) and PS mice (black). Data are expressed as scatter dot plot with the mean (n=9–10). Statistical analysis was performed using Mann-Whitney test. **P<0.01, significantly different from the corresponding control group. (C) VMR to colorectal distensions in response to increasing pressures of distension (15, 30, 45 and 60 mm Hg) was measured in both M and FM PS offspring. Measurements were done before (white) and after intracolonic administrations of C14AsnGABA (black). Data are expressed as mean±SEM (n=19 mice/group, two independent experiments). Statistical analysis was performed using two-way analysis of variance and subsequent Sidak multiple comparison test. *P<0.05, ***P<0.001, ****P<0.0001 significantly different from the pretreatment group. The results are also expressed as area under the curve (AUC) presented as scatter dot plot with the mean for M (square) and F (circle) mice. statistical analysis was perform using a Wilcoxon test. ****p<0.0001, significantly different from the pretreatment group. F, female; GABA, LC-MS/MS, iquid chromatography–tandem mass spectrometry; M, male; ns, not significant; PS, prenatal stress; VMR, visceromotor response.