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. 2023 Apr 3;27(5):104. doi: 10.3892/mmr.2023.12991

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Principle of digital PCR. This figure was drawn using Figdraw software online (https://www.figdraw.com/static/index.html). The process of digital PCR mainly includes sample preparation, sample distribution, PCR amplification, and fluorescence signal detection and analysis. The EP tube contains nucleic acid extracted from the sample to be tested. First, the sample to be tested was microdropped to make ~20,000 small droplets of water in oil, and then the microdrop system was amplified. Finally, the fluorescence signal of each microdrop was detected, and the data was analyzed.