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. 2023 Apr 3;27(5):104. doi: 10.3892/mmr.2023.12991

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 8. — Principle of sequencing technologies. (A) Next-generation sequencing. (B) Third-generation Sequencing. This figure was drawn using biorender software online (https://app.biorender.com/). (A) Next-generation sequencing uses fluorescence with different colors to label four different dNTPs. When the complementary strand is synthesized by DNA polymerase, different fluorescence is released with each addition of dNTP. The sequence information of the DNA to be tested is obtained by analyzing the captured fluorescence signal. (B) Third-generation sequencing uses electrophoresis technology to drive a single nucleic acid molecule through a nanopore and identifies the nucleic acid sequence by detecting the change in current caused by different bases of nucleic acid passing through the nanopore.