Figure 6.

Effects of the ERα-selective agonist, PPT, for 2 and 4 h on the expression and localization of GAL-3 in DU-145 cells. Cells were incubated in the absence (control, C) or presence of ERα-selective agonist PPT (10 nM) for 2 h and 4 h at 37°C. Cells were also untreated or pre-treated with ERα-selective antagonist MPP (10 nM) for 30 min. Incubation was continued in the absence or presence of PPT (10 nM) for 4 h at 37°C. Immunostaining for GAL-3 (red) was detected as described in the Materials and methods. Nuclei were stained with DAPI (blue). Negative control was performed in the absence of primary antibody (insert). Scale bars, 20 µm. Images are representative of four independent experiments. ER, estrogen receptor; PPT, 4,4′,4”-(4-propyl-(1H)-pyrazole- 1,3,5-triyl)trisphenol; DPN, 2,3-bis(4-hydroxyphenyl)-propionitrile; GAL-3, galectin-3; MPP, 1,3-bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride.