Figure EV2. Lipid metabolism features of POI.

• A
  Heatmap showing the top 25 differentially abundant metabolites in the ovaries of mice on a low BCAA diet. Control, N = 10; low BCAA, N = 8.
• B
  The relative abundance of BCAAs in the ovaries of mice on a low BCAA diet. Control, N = 10; low BCAA, N = 8; Boxplot, central band stands for median, boxes stand for 50% of the data, and whiskers stand for min or max of the data.
• C
  GSEA of the proteomics data in the serum of the Fudan Cohort. N = 11.
• D
  GSEA of RNA‐seq data from the liver in the mice on a low BCAA diet or control diet. N = 6.
• E
  The PCA of serum from mice on a low BCAA diet or control diet. N = 10.
• F
  The relative cell viability of KGN cells with ceramide treatment. N = 6.
• G
  The percentage of Annexin V positive cells of KGN cells with ceramide treatment. N = 3.
• H–J
  The pregnancy rate, body weight, and food intake from mice with ceramide treatment. N = 10.
• K
  The relative abundance of ceramide in the serum of mice with myriocin treatment. N = 3; Truncated violin plot, central band stands for median, and dotted lines stand for the upper quartile or the lower quartile of the data.
• L
  The serum concentration of FSH in mice with ceramide treatment. N = 8.
• M
  The changes in follicle count from mice with ceramide treatment. N = 8.

Data information: S1, Primordial; S2, Primary; S3, Secondary; S4, Antral; S5, Atretic. Error bars stand for SEM. The P‐value was calculated by a two‐tailed t‐test with 2‐way ANOVA correction.

Source data are available online for this figure.