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. 2023 Feb 27;15(4):e17450. doi: 10.15252/emmm.202317450

Figure 4. BCAA‐induced POI via ceramide‐ROS axis.

Figure 4

  • A
    GSEA results of RNA‐seq data. N = 5.
  • B
    sNuc‐seq data of ovarian nonimmune cells. Granulosa cells (cluster 0), mesothelial cells (cluster 1), thecal cells (cluster 2), and endothelial cells (cluster 3).
  • C, D
    (C) Relative expression of genes related to ROS in granulosa cells and (D) the concentration of E2 secreted by KGN cells with H2O2 treatment. N = 3.
  • E, F
    Relative abundance of GSH and GSSG. N = 3.
  • G
    The concentration of E2 secreted by KGN cells with ceramide treatment w/o NAC. N = 4.
  • H–J
    The serum concentration of FSH, AMH, and E2. (H) Control, N = 10; BCAA, N = 5; TG = 9; TG + BCAA = 8; (I) Control, N = 5; BCAA, N = 5; TG = 6; TG + BCAA = 6; (J) N = 5.
  • K
    The changes in follicle count. Control, N = 5; BCAA, N = 4; TG = 5; TG + BCAA = 5.
  • L
    Body weight. Control, N = 5; BCAA, N = 5; TG = 5; TG + BCAA = 4.
  • M
    Pregnancy rate. N = 4.
  • N
    Number of pups from mice with TG or TG + BCAA supplement treatment. N = 4.

Data information: Error bars stand for SEM of biological repeats. The P‐value was calculated by a two‐tailed t‐test with 2‐way ANOVA correction.

Source data are available online for this figure.