Figure EV4. BCAA supplement protected the granulosa cells from ROS inducer.

• A
  Heatmap showing the relative abundance of metabolites in KGN cells treated with ceramides. N = 3.
• B
  The concentration of E2 secreted by KGN cells with H₂O₂ treatment w/o BCAT2 inhibitor. N = 5.
• C
  The concentration of E2 secreted by KGN cells with ceramide treatment w/o BCAT2 inhibitor. N = 5.
• D
  Elevation of ceramide in the serum of TG‐treated mice. N = 5.
• E
  BCAA supplement prevented the elevation of FSH in mice with 3‐NPA treatment. Control, N = 7; BCAA, N = 5; 3‐NPA, N = 9; 3‐NPA + BCAA, N = 16.
• F
  BCAA supplement rescued the decrease of primordial follicles in mice with 3‐NPA treatment. Control, N = 3; BCAA, N = 3; 3‐NPA, N = 5; 3‐NPA + BCAA, N = 4.

Data information: S1, Primordial; S2, Primary; S3, Secondary; S4, Antral; S5, Atretic. Error bars stand for SEM. The P‐value was calculated by a two‐tailed t‐test with 2‐way ANOVA correction.

Source data are available online for this figure.