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. 2023 Feb 6;15(4):e16128. doi: 10.15252/emmm.202216128

Figure EV1. Dynamin and Clathrin inhibitors do not affect internalization of VE‐PTP under shear stress.

Figure EV1

  • A
    HUVECs were treated either with DMSO (vehicle) or with Hydroxy‐Dynasore at 100 μM for 1 h followed by exposure to shear stress of 15 dyn/cm2 for 5 to 30 min. Subsequently, cells were fixed, permeabilized, and stained for VE‐PTP (red), VE‐cadherin (green) and for nuclei (blue).
  • B
    HUVECs were treated with vehicle or Hydroxy‐Dynasore as in (A), followed by incubation under static conditions with transferrin‐Alexa 488 (green) at 20 μg/ml for 15 min. Non‐internalized transferrin was removed with acetic buffer pH 2.0.
  • C, D
    Similar as (A and B), except that Hydroxy‐Dynasore was replaced by Pitstop‐2 at 30 μM.

Data information: Scale bars: 20 μm.