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. 2023 Feb 6;15(4):e16128. doi: 10.15252/emmm.202216128

Figure EV3. Tie2 is required for shear stress‐induced export of nuclear FoxO1.

Figure EV3

  1. HUVECs treated either with control siRNA or Tie2 siRNA were exposed to 15 dyn/cm2 shear stress for 5 to 30 min, followed by fixation, permeabilization, and staining for FoxO1 (red), VE‐cadherin (green) and nuclei (blue). Scale bar: 20 μm.
  2. Amount of nuclear FoxO1 staining. Mean ± SEM, n = 6 cultures on independent flow chamber lanes. ***P < 0.001, by Bonferroni's test. P = 0.17 (0 min), 0.00000090 (5 min), 0.14 (10 min) and 0.31 (30 min).