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. 2022 Jul 22;25:766–782. doi: 10.1016/j.bioactmat.2022.07.002

Fig. 5.

Fig. 5

Functional recovery after AP-EXOR&L&S treatment in contused SCI mice. Adult mice were subjected to spinal cord contusion and received intravenous injections of AP-EXO or AP-EXOR&L&S beginning at day 1 after contusion at a dose of 1 mg/kg/week for 5 weeks. The BMS scores of mice were then determined weekly for 6 weeks. Four weeks after injury, BDA was intra-cortically injected to trace CST axons. One day before tissue extraction, gait analysis of the mice was performed. (a–c) BMS scores of spinal-cord-contused animals treated with AP-EXO or AP-EXOR&L&S (sham: n = 9, SCI: n = 15, AP-EXO: n = 21, AP-EXOR&L&S: n = 21, sham group designated as the mice received laminectomy without contusion injury, SCI group designated as mice injected with PBS, AP-EXO group designated as mice injected with autologous mouse exosomes, and AP-EXOR&L&S group indicated the mice injected with peptide-painted exosomes). a, BMS score assessment over time (*p < 0.05, #p < 0.05, one-way ANOVA on ranks post Dunn's Method, * designated as comparison between the SCI group and the AP-EXOR&L&S group, # designated as comparison between the SCI group and the AP-EXO group). b, BMS score at week 6 after spinal cord contusion (*p < 0.05, one-way ANOVA on ranks post Dunn's Method). c, percentage of animals at successive score segments after treatment. (d–j) Catwalk gait analysis to determine the locomotor recovery after AP-EXO or AP-EXOR&L&S treatment (sham: n = 10, SCI: n = 10, AP-EXO: n = 16, AP-EXOR&L&S: n = 16). d, representative images of footprints after AP-EXOR&L&S treatment. e, swing duration for fore-and hind-limb after AP-EXO or AP-EXOR&L&S treatment (*p < 0.05, one-way ANOVA on ranks post Dunn's Method). f, base of support for fore-and hind-limbs (*p < 0.05, one-way ANOVA on ranks post Dunn's Method). g, stride length of the left and right forelimb (*p < 0.05, one-way ANOVA post Student-Newman-Keuls test). h, stand time of left and right hind-limbs (*p < 0.05, one-way ANOVA on ranks post Dunn's Method). i, maximum contact areas of left and right hind-limb (*p < 0.05, one-way ANOVA on ranks post Dunn's Method). j, average swing speed of the left and right hind-limbs (*p < 0.05, one-way ANOVA on ranks post Dunn's Method). (k) Examples of motor-evoked potential (MEP) recordings from mice at week 6 post-surgery.