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. 2022 Jul 22;25:766–782. doi: 10.1016/j.bioactmat.2022.07.002

Fig. 7.

Fig. 7

Systemic evaluation of HP- EXOR&L&S in nude mice with spinal cord contusion. Nude mice were subjected to spinal cord contusion and then intravenously injected with HP-EXO or HP-EXOR&L&S at a dose of 1 mg/kg/week for 5 weeks starting at day 1 after contusion. BMS scores were assessed over time. Gait analysis was conducted 1 day before tissue collection. (a, c-h, sham: n = 5, SCI: n = 8, HP-EXO: n = 8, HP-EXOR&L&S: n = 8). (a) BMS scores of contused nude mice treated by HP-EXO or HP-EXOR&L&S (*p < 0.05, #p < 0.05, one-way ANOVA on ranks post Tukey Test, **p < 0.001, ##p < 0.001, one-way ANOVA post Student-Newman-Keuls test, *dominated as comparison between the SCI group and the HP-EXOR&L&S group, # denominated as comparison between the SCI group and the HP-EXO group). (b–f) Gait analysis of mice treated with HP-EXO or HP-EXOR&L&S. b, representative footprint images of contused mice treated with PBS, HP-EXO or HP-EXOR&L&S. c, base of support for forelimbs and hindlimbs after treatment (*p < 0.05, **p < 0.001, one-way ANOVA post Student-Newman-Keuls test). d, stride length of the left and right forelimbs (**p < 0.001, one-way ANOVA post Student-Newman-Keuls test, *p < 0.05, one-way ANOVA on ranks post Dunn's Method). e, stand time of left and right hindlimb (*p < 0.05, one-way ANOVA on ranks post Dunn's Method). f, max contact area of left and right hindlimb (*p < 0.05, **p < 0.001, one-way ANOVA post Student-Newman-Keuls test). (g) measurement of plasma ALP, ALT and AST enzymes in sham controls, PBS treated SCI controls, and SCI mice treated with HP-EXO or HP-EXOR&L&S. (h) measurement of plasma BUN, CREA or UA in sham controls, PBS treated SCI controls, and SCI mice treated with HP-EXO or HP-EXOR&L&S. (i) hematoxylin and eosin (H&E) staining of kidney, liver, lung and spleen tissue sections from sham controls, PBS treated SCI controls, and SCI mice treated with HP-EXO or HP-EXOR&L&S. Scale bar:100 μm. (j) Immunohistochemistry for CD3-positive T lymphocytes and CD68-positive macrophages in the kidney and liver from sham controls, PBS treated SCI controls, and SCI mice treated with HP-EXO or HP-EXOR&L&S. Scale bar: 100 μm.