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. 2023 Mar 5;19(5):1543–1563. doi: 10.7150/ijbs.77133

Figure 5.

Figure 5

CircRBM33 significantly increases mitochondrial respiration in PCa cells. (A) KEGG pathway analysis of FMR1-RIP sequencing. (B) qRT-PCR determined the RNA expression levels of the key molecules involved in pyruvate metabolism, the pentose phosphate pathway, glycolysis/gluconeogenesis, and the citrate cycle in PCa cells when circRBM33 was upregulated or downregulated. (C) WB detects the PDHA1 expression differences in PCa cells after circRBM33 is overexpressed or silenced. (D-E) Detection of the changes in ATP production and acetyl-CoA levels in circRBM33-silenced and circRBM33-overexpressed cells compared to negative control cells. (F) Detection of the variation in the NAD+/NADH ratio in circRBM33-silenced and circRBM33-overexpressed cells compared to their own negative control cells. (G) Seahorse experiments detect the OCR in circRBM33-silenced and circRBM33-overexpressed cells in comparison to their own negative control cells.