Figure 6.

Neutrophil depletion prevents gastric cancer cell tumorigenesis through inhibiting tumor EMT in tumor-bearing mice. (A) Treatment with anti-Ly6G antibody decreased the numbers of peripheral neutrophils significantly compared with control IgG treatment (P = 0.000). (B, C) The subcutaneous allograft models study showed that tumor volumes in anti-Ly6G-treated group were smaller than that in IgG-treated group. (D, E, F) Further assays revealed that E-cad level was increased whereas expressions of Vim, Snail and p-JNK were decreased significantly in anti-Ly6G-treated group with relative to IgG-treated group (dotted line, necrotic area in tumor). (G, H) The abdominal allograft models also showed E-cad was up-regulated while Vim was down-regulated in anti-Ly6G-treated group compared with IgG-treated group. Tumor cells were spindle in IgG-treated group, and tumors invaded into liver and the liver envelope structure was destroyed (white triangular arrow), whereas tumor cells were polygonal in anti-Ly6G-treated group, and tumor was not found to invade into liver and the liver envelope was intact (white triangular arrow). (**, P < 0.01; ***, P < 0.001).