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. 2023 Feb 27;19(5):1471–1489. doi: 10.7150/ijbs.77979

Figure 4.

Figure 4

HSP90 is probably responsible for Tim-AIII-induced ferroptosis in NSCLC cells. A The most likely predicted targets of Tim-AIII in HSP90. B. SPR analysis of Tim-AIII binding to wild type HSP90 (Kd =28.2 μmmol/L). C SPR analysis of Tim-AIII binding to mutant HSP90 (Kd=104.6 μmmol/L) D The protein expression of HSP90 was examined by WB after treatment with Tim-AIII for 48 h in H1299 and A549 cells. E The expression of HSP90 were examined by RT-PCR after treatment with Tim-AIII for 48 h in H1299 and A549 cells. F H1299 and A549 cells were co-treated with Tim-AIII with or without the HSP90 classic inhibitor (tanespimycin) for 48 h, and cell vitality was assayed by the CCK-8 assay. Quantitative data were presented as mean ± SD. *p< 0.05, **p < 0.01, ***p < 0.001.