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. 2023 Feb 27;19(5):1471–1489. doi: 10.7150/ijbs.77979

Figure 6.

Figure 6

Ferroptosis induced by the Tim-AIII was dependent on HSP90. A The transfection efficiencies of HSP90-shRNA in H1299 and A549 cells were evaluated by observing the fluorescence of GFP (200X). B The expression of HSP90 protein in H1299 and A549 cells after transfection was detected by WB. C H1299 and A549 cells were transfected with control-shRNA and HSP90-shRNA, followed by Tim-AIII (4 μM) treatment for 48 h, and cell vitality was assayed by the CCK-8 assay. D H1299 and A549 cells were transfected with control-shRNA and HSP90-shRNA, followed by Tim-AIII (4 μM) treatment for 48 h, and the OD value of LDH was assayed. E-G H1299 and A549 cells were transfected with control-shRNA and HSP90-shRNA, followed by Tim-AIII (4 μM) treatment for 48 h, and the level of intracellular iron, MDA and GSH were assayed. H H1299 and A549 cells were transfected with control-shRNA and HSP90-shRNA, followed by Tim-AIII (4 μM) treatment for 48 h, the GPX4, HSP90 and the ubiquitination level of GPX4 were analyzed by WB assay with anti-Ubiquitination antibody. Quantitative data were presented as mean ± SD. *p< 0.05, **p < 0.01, ***p < 0.001.