Fig. 5.

Immunofluorescence and immunohistochemistry after Exos-miRNA 24-3p treatment. CK3, ZO-1, EGFR and MMP9 of the corneal epithelial defect models were treated with PBS, Exos, Exos-miRNA NC and Exos-miRNA 24-3p on (a and b) days 7 and (c and d) 12. Nuclei (blue) were labeled with DAPI in the merge image of c. Immunofluorescence or immunohistochemical semi-quantitative analysis on day 7 (b) or 12 (d) was performed using imageJ (n = 5). ep: epithelium, st: stroma. *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001 for native cornea; #p ≤ 0.05, ##p ≤ 0.01 and ###p ≤ 0.001 for control; &p ≤ 0.05, &&p ≤ 0.01 and &&&p ≤ 0.001 for PBS. Scale bar: 50 μm.