Figure S7. Analysis of ChrRNA-seq in wt and vtRNA1-2 KO cells.
(A) Western blot of chromatin and whole-cell lysate fraction components isolated for ChrRNA sequencing. (B) Principal component analysis plot shows three biological replicates corresponding to the samples from HEK293T cells in WT and vtRNA1-2 KO conditions. The RNA composition of cells in the WT condition differed significantly from those in the vtRNA1-2 KO condition. (C) Box plot showing log2-transformed read counts in three biological replicates per condition. (D) Pie chart showing gene-type distribution of down-regulated genes in vtRNA1-2 KO cells. (E) As in (D), for up-regulated genes.