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Evaluations for the osteogenic differentiation of BMSCs in the presence of various scaffolds. (A) ALP staining and (B) quantified ALP activity for BMSCs cultured for 7 and 14 days. (C–E) Relative expressions of three osteogenic-related genes (BMP-2, COL-I, and OCN) detected by qPCR at day 5. *P < 0.05, significant, **P < 0.01 and ***P < 0.001, highly significant (n = 3).
