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Histological, immunohistological, and immunofluorescence staining for the evaluation of bone regeneration. (A) H&E staining and Masson's trichrome staining for sections collected at 8 W post-operation. (B) Immunohistochemical staining of TRAP and OCN expression at 8 W post-operation. (C) Immunofluorescence staining of CD31 (red), CGRP (green), and DAPI (blue) expression at 4 W and 8 W post-operation.
