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. 2022 Aug 24;23(1):131–144. doi: 10.1111/1755-0998.13698

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© 2022 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Histograms displaying the distribution of spearman correlation coefficients of cytosine methylation status with age following filtering of invariant sites across CpG, CHG, and CHH‐methylation contexts from 24 loblolly pine trees of differing ages. (a) of 21,567 CpGs analysed, 533 (2.5%) showed correlations between methylation status and age of R > |.5|. (b) of 25,501 CHGs analysed, 869 (3.4%) displayed correlations between methylation status and age of R > |.5|. (c) of 33,151 CHHs analysed, 308 (0.93%) showed correlations between age and methylation status of R > |.5|.