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. Author manuscript; available in PMC: 2023 Apr 11.
Published in final edited form as: J Med Chem. 2021 May 19;64(11):7617–7629. doi: 10.1021/acs.jmedchem.1c00318

Figure 2.

Figure 2.

Representative gels of the TOP1-mediated DNA cleavage assay. Lane 1, DNA alone; lane 2, DNA and TOP1; lane 3, DNA and TOP1 with CPT (1 μM); lane 4, DNA and TOP1 with LMP744 (1 μM); lanes 5–28, DNA and TOP1 with the tested compounds at 0.1, 1.0, 10, and 100 μM concentrations, respectively. The arrows and numbers at left indicate the cleavage site positions.