Fig. 10.

Evaluation of compound 1 on Pseudomonas virulence in vivo. (A) The efficacy of compound 1 against P. aeruginosa was tested with embryos injured in the tail fin and bath infected with PAO1 suspension at approximately 8.10⁷ CFU·mL⁻¹ in the presence of compound 1 at 20 μm (in 0.1% DMSO). Injured embryos in the control group were treated with PAO1 suspension in the presence of DMSO at 0.1%. A significant difference (P < 0.01) is found in the survival curve of treated versus non‐treated embryos. (B) The toxicity of compound 1 at 20 μm was monitored after immersion of embryos injured in the tail fin. For all experiments, the embryo survival was monitored for 45 h and survival curves were represented with a Kaplan–Meier representation. Graphs represent the pool of three minimum independent experiments (n = 120 larvae for compound 1 test or 60 larvae for toxicity test, in total per condition). ** means P value <0.01, ns means no significant difference.