Fig. 2.

Mechanical inoculation, detection and seed transmission of Arabidopsis latent virus 1 (ArLV1). (a) Nicotiana benthamiana plants at 2 wk after mechanical inoculation with two isolates of ArLV1 – ArLV1_A (left photo) and ArLV1_B (middle photo) – both showing ArLV1‐induced leaf mottling symptoms (indicated with arrowheads). The right photo shows a control plant mock inoculated with buffer. (b) Quantitative reverse transcription polymerase chain reaction (RT‐qPCR) detection of RNA1 and RNA2. qPCR detection of ArLV1 in N. benthamiana was repeated as a positive control every time we inoculated Arabidopsis thaliana, with comparable results. (c) ArLV1_A in infected A. thaliana leaves, visualized by transmission electron microscopy. Arrowheads indicate the viral particles (Bar, 100 nm). (d) Percentage of infected progeny from A. thaliana Col‐0 parent plants infected with the isolate ArLV1_A or ArLV1_B. Infection was detected by RT‐qPCR on leaf material (Figs S1, S2).