Figure 1.
Identification of exosomes derived from EPCs (A) Immunofluorescence analysis of characteristic cell markers of EPCs. Green or red color represented measured markers (VEGFR2, CD34 and CD133, respectively) and blue color represent nucleus. (B) Immunofluorescence analysis of EPCs with fluorescence labeled Dil-ac-LDL and Fitc-UEA-1. (C) Representative micrographs showing exosome ultrastructure. Scale bar, 500 nm. (D) Exosome size distribution of EPC-EXOs. (E) Western blotting of EPC-EXOs surface marker protein expression levels. (F) Uptake of EPC-EXOs by HaCaTs was detected by PKH26 and PKH27. Scale bar, 20 μm.