Table 2.
Dissociation constants for oNPC and cellobiose binding to TrCel7A WT and catalytic mutants and PcCel7D from fluorescence titration experiments, and inhibition constants for cellobiose a and lactose.
| Enzyme | K d for oNPC (μm) | K d for cellobiose (μm) | K i for cellobiose a (μm) | K i for lactose (μm) b |
|---|---|---|---|---|
| TrCel7A WT | 7.4 ± 0.4 | 23 ± 4 | 24 c | 180 ± 16 |
| TrCel7A D214N | 7.1 ± 0.7 | 8.9 ± 1.1 | – | – |
| TrCel7A E212Q | 4.7 ± 0.4 | 8.1 ± 0.3 | – | – |
| TrCel7A E217Q | 3.9 ± 0.4 | 14 ± 3 | – | – |
| PcCel7D | 110 ± 10 | – | 180 d | 183 ± 16 |
a
Previously published inhibition constants from [13]
b
The error margin represents the 95% confidence interval of the profile likelihood from graphpad prism 8
c
Competitive inhibition constant from inhibition experiments with pNPL as substrate at 30 °C, pH 5.0 [13]
d
Mixed‐type inhibition constant (α = 5.7) estimated from inhibition experiments with CNP‐Lac (2‐chloro‐4‐nitrophenyl‐β‐lactoside) as substrate at 33 °C, pH 5.5 [13].