Fig. 2.
(A) Intracellular distributions of Mito-ZS, Lyso-ZS and ER-ZS (red, λex = 580 nm, λem = 600–700 nm) by CLSM observation, wherein mitochondria, lysosomes and endoplasmic reticulum were stained by commercial dyes (λex = 488 nm, λem = 510–530 nm) of Mito-Tracker Green, Lyso-Tracker Green or ER-Tracker Green, respectively. Scale bars: 20 μm. (B) ROS detection in 4T1 cells using DCFH-DA as the fluorescence indicator. Blue fluorescence (λex = 405 nm, λem = 450–470 nm); green fluorescence (λex = 488 nm, λem = 510–530 nm). Scale bars: 20 μm. (C) The quantified fluorescence intensities of DCFH-DA. (D) Fluorescence intensities of DCFH-DA as a function of Mito-ZS concentration upon excitation at 580 nm (40 mW/cm2, 5 min, 12 J/cm2).