Fig. 3.

(A) Representative morphologies of HeLa and 4T1 cells under treatment of diverse organelle-targeting photosensitizers (5 μM) in presence or absence of excitation (580 nm, 40 mW/cm², 10 min, 24 J/cm²). Scale bars: 20 μm. (B) Annexin V-FITC/PI co-staining assay, red fluorescence of PI (λ_ex = 488 nm, λ_em = 650–750 nm) and green fluorescence of Annexin V (λ_ex = 488 nm, λ_em = 510–530 nm). Scale bars: 20 μm. (C) Quantification of LDH release. (D) Cell viabilities of 4T1 cells under treatment of diverse organelletargeting photosensitizers (0–4 μM) in presence or absence of excitation (580 nm, 40 mW/cm², 10 min, 24 J/cm²). (E) Dark cytotoxicities of a variety of organelle-targeting photosensitizers against 4T1 cells. (*p < 0.05, **p < 0.01, ***p < 0.001.)