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. 2022 Aug 2;25:580–593. doi: 10.1016/j.bioactmat.2022.07.016

Fig. 5.

Fig. 5

(A) Identification of translocation of CRT to plasma membrane by CLSM. Scale bars: 20 μm. (B) Quantification of CD80 and CD86 by flow cytometry. (C) Quantified fluorescence intensities of CRT based on the representative CLSM images in (A). (D) Quantification of the extracellular levels of ATP for 4T1 cells upon PDT. (E–H) Quantification of the Secretion of IL-12p70, IL-6, TNF-α and IL-4 by ELISA assay (n = 3). The error bars (n = 3) represent means ± SD. (*p < 0.05, **p < 0.01, ***p < 0.001.) The 4T1 cells under treatment with the diverse organelle-targeting photosensitizers (5 μM) in presence or absence of excitation (580 nm, 40 mW/cm2, 10 min, 24 J/cm2).