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. 2022 Sep 11;290(3):763–779. doi: 10.1111/febs.16614

Fig. 2.

Fig. 2

G6PD knockdown impaired tumorigenesis of breast cancer. (A) G6PD knockdown efficiency was validated on G6PD gene and protein expression in MDA‐231 and MCF‐7 cells by q‐PCR and Western blotting, respectively. (B) Immunoblot analysis of the changes in G6PD, cl‐caspase3, and cl‐PARP in MDA‐231‐shG6PD and MCF‐7‐shG6PD cells. (C) Viability of MDA‐231‐shG6PD and MCF‐7‐shG6PD cells was measured by MTT assay following 24 h. (D) Cell death of MDA‐231‐shG6PD and MCF‐7‐shG6PD cells was determined with the PI exclusion assay following 24 h. (E, F) The effect of G6PD knockdown in MDA‐231 and MCF‐7 cells was investigated by colony formation assay. Images were taken with 4× magnification (E) and Transwell assay for cell migration. Images were taken with 40× magnification (F). (G) Lung colonization was measured using bioluminescence at 0, 6, and 24 h after injection (n = 5). Luminescence colour scale: 0 h (min = 1 × 106, max = 1 × 107) and 6 and 24 h (min = 2 × 105, max = 2 × 106). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.