Fig. 3.

Pharmacological inhibition of G6PD by 6‐AN blocked cell survival, migration and colony formation. (A) MDA‐231 and MCF‐7 cells were treated with different concentrations of 6‐AN for 24 h. cell viability was measured using MTT. (B) MDA‐231 and MCF‐7 cells were treated with different concentrations of 6‐AN for 24 h. Immunoblot analysis of the protein changes in G6PD, cl‐caspase 3, and cl‐PARP. (C) MDA‐231, MCF‐7, SKBR3, and 293T cells were treated with 6‐AN (10 μm) for 24 h, and cell viability was measured with MTT. (D) Cells were treated with 6‐AN for 48 h. Cell death was determined with the PI assay. (E) Cells were treated with 6‐AN for 24 h. Gene expression of G6PD was measured using q‐PCR. (F) Cells were treated with 6‐AN for 2 or 24 h. G6PD was detected by the enzyme activity kit. (G, H) The effect of 6‐AN on the migration and colony formation was investigated by Transwell assay. Images were taken with 40× magnification (G) and colony formation assay. Images were taken with 4× magnification (H). (D–H) 6‐AN was used 20 μm for MDA‐231 and 30 μm for MCF‐7. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.