FIGURE 2.

Effect of temozolomide (TMZ) treatment in U251 and U251R cell lines. Protein expression and calibration after pretreatment with (A) SB203580 (10 μM) and (B) U0126 (10 μM) followed by TMZ (100 μM) treatment for 24 h in U251 and U251R cells; both SB203580 and U0126 did not enhance U251R cell apoptosis after TMZ treatment. (*p < .05, **p < .01, ***p < .001, compared with U251 control cells; #p < .05, ##p < .01, ###p < .001, compared with U251R control cells). GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; MGMT, methyl guanine methyltransferase