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High‐performance liquid chromatography (HPLC). (A) HPLC analysis of 50 μM thymoquinone (TQ) standard solution; TQ retention time: 26.345 min. (B) HPLC analysis of brain extract samples of mice with 1 h TQ intraperitoneal (IP)‐injection; TQ retention time: 26.769 min. The results suggest that TQ exists in mice brain tissue.
